منابع مشابه
Selectable Markers for Gene Therapy
One of the major problems with current approaches to gene 9 therapy is the instability of expression of genes transferred into 10 recipient cells. Although in theory, homologous recombination 11 or use of artificial chromosomes can stabilize sequences with 12 wild-type regulatory regions, such approaches to gene therapy 13 are not yet feasible and may not be efficient for some time to 14 come. ...
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As a result of gene targeting, selectable markers are usually permanently introduced into the mammalian genome. Multiple gene targeting events in the same cell line can therefore exhaust the pool of markers available and limit subsequent manipulations or genetic analysis. In this study, we describe the combined use of homologous and CRE-loxP-mediated recombination to generate mouse embryonic st...
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Plastid transformation is widely used in basic research and for biotechnological applications. Initially developed in Chlamydomonas and tobacco, it is now feasible in a broad range of species. Selection of transgenic lines where all copies of the polyploid plastid genome are transformed requires efficient markers. A number of traits have been used for selection such as photoautotrophy, resistan...
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T he genomes of higher eukaryotes encode not only proteins but also diverse noncoding RNAs, particularly small (20to 30-nt) regulatory RNAs (1–3). The small RNAs include microRNAs (miRNAs), siRNAs, and piwi-interacting RNAs (piRNAs) (4, 5). These small RNAs repress gene expression at the transcriptional or posttranscriptional levels and have critical functions in genome defense, growth, develop...
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Calcium waves in membrane fusion hagolysosome fusion is a calcium dependent process. Now, Randall Worth and colleagues (University of Pennsylvania School of Medicine, Philadelphia, PA), and Howard Petty and colleagues (University of Michigan School of Medicine, Ann Arbor, MI) find that a three amino acid motif in the IgG receptor Fc RIIA causes a calcium wave traveling along the plasma membrane...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2020
ISSN: 1548-7091,1548-7105
DOI: 10.1038/s41592-019-0713-3